ABSTRACT
The chemical properties of characteristic components are significant to the manufacturing quality control of big brand traditional Chinese medicine. In this study, the Huangjing Zanyu Capsules were used as the research carrier to determine the content of five characteristic components including icraiin, emodin, schisandrin A, 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, and osthole simultaneously by high-performance liquid chromatography(HPLC). The results showed that the chemical properties of five cha-racteristic components had a good linear relationship(r>0.999 9) within the quantitative range; the relative standard deviations(RSD) was 0.11%-2.0% and 0.25%-2.8% respectively for intra-day and inter-day precision; the RSD of repeatability was 1.8%-2.6%; the RSD of stability within 48 hours was 0.19%-2.8%, and the average recovery rate was 95.52%-100.1%, all meeting the requirements of pharmaceutical quantitative analysis. Additionally, the interval estimation method was used to directly reflect the distribution of samples with abnormal chemical properties of characteristic components, and the results showed ten samples were detected beyound the 95% control line of confidence level. Multivariate statistical process control(MSPC) method was used to monitor the abnormal samples of Huangjing Zanyu Capsules collectively, and the results showed that two samples were beyond the 95% control line of Hotelling's T~2 and three samples beyond the 95% control line of squared prediction error(SPE), indicating consistent quality control of Huangjing Zanyu Capsules. In conclusion, the proposed method is not only accurate and efficient but also a compensation for the traditional single-component quality control method, providing a scientific basis for the quality control in manufacturing process of Huangjing Zanyu Capsules. Furthermore, it could also serve as a reference method for the quality control in manufacturing big brand traditional Chinese medicine.
Subject(s)
Capsules , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Medicine, Chinese Traditional , Quality ControlABSTRACT
Objective:To study on the differences of profile spectra among chemical components in Magnoliae Officinalis Cortex from different varieties and habitats,and to screen and identify the characteristic components affecting the quality difference of this herb. Method:The chromatogram data sets of Magnoliae Officinalis Cortex from different varieties and habitats were obtained by liquid chromatography-time-of-flight-mass spectrometry(LC-TOF-MS).Principal component analysis,partial least squares-discriminant analysis and cluster analysis were used to compare the differences in chemical profiles among Magnoliae Officinalis Cortex from different varieties and habitats,and adopted to screen out the characteristic chemical constituents that resulted in these differences and to perform mass spectrometry analysis and comparison. Result:Eleven characteristic peaks were identified by LC-TOF-MS chromatographic data and reported in the literature.The use of chemical profile could distinguish different habitats of Magnoliae Officinalis Cortex,but could not completely distinguish different varieties of this herb. Conclusion:LC-TOF-MS can easily and quickly study on the profile differences of chemical substances in Magnoliae Officinalis Cortex from different varieties and different habitats,the results of this study can provide a theoretical basis for the quality evaluation and pharmacodynamic material basis of this herb.
ABSTRACT
Thirty-two batches of cultivated and wild Glycyrrhiza uralensis were obtained from three geographical regions. Comparative study of water characteristic components of G. uralensis from three geographical origins was conducted by PCA,OPLS-DA chemical pattern recognition combined with LC-TOF/MS and muti-component analysis. The similarity of fingerprints of 32 batches of medicinal materials ranged from 0. 903 to 0. 999. Patterns recognition could be used to distinguish cultivated G. uralensis in Gansu and Xinjiang areas from cultivated and wild plants in Inner Mongolia. Then a total of thirty-one common constituents were identified by LC-TOF/MS analysis coupled with standard compounds information. The contents of four flavonoid glycosides and five saponins were determinated by HPLC and compared using One-way ANOVA. The results showed that there was no significant difference in the contents of 5 triterpenoid saponins among the three regions,but the contents of 4 flavonoid saponins showed the trend of Inner Mongolia >Gansu≈Xinjiang( P<0. 05). In the same Inner Mongolia region,the contents of 4 flavonoid glycosides and 5 triterpenoid saponins in wild plant was significantly higher than that in cultivated plants( P<0. 01). In addition,the contents of liquiritin,isoliquiritin,licorice-saponin A_3,22β-acetoxyl-glycyrrhizic acid and uralsaponin B in Gansu and Xinjiang were obviously lower than those in Inner Mongolia,but the contents of glycyrrhizic acid,the main component of G. uralensis,were not different in the three geographical regions. In Inner Mongolia,the contents of liquiritin,isoliquiritin,licorice-saponin A_3,licorice-saponin G_2 and glycyrrhizic acid in wild plants were significantly higher than those in cultivated plants. In conclusion,qualitative/quantitative analysis of multi-index components combined with pattern recognition could effectively evaluate the quality of cultivated and wild licorice in different regions. It was helpful for us to understand the reality of licorice in different regions,and provided scientific basis for the development and comprehensive utilization of licorice resources.
Subject(s)
China , Geography , Glycyrrhiza uralensis , Chemistry , Glycyrrhizic Acid , Plant Extracts , Saponins , WaterABSTRACT
AIM:To study characteristic components and fingerprint of the essential oils of processed Rhizoma curcumae longae,and further use as one of evaluation of quality control. METHODS:The essential oils were ex-tracted by steam distillation from ten batches of samples,and the characteristic components of the essential oils were analysed by GC-MS. And then mutual fingerprint of the characteristic components was established by Microsoft Office Excel 2003. RESULTS:Twenty-seven characteristic components of essential oil consisted of Ar-turmerone,Tumerone,Curlone,?-Curcumene,?-Zingiberene,?-Sesquiphellandrene and their derivatives,which were compose of about 88. 8% ～94. 9% of the total essential oil. The retention time and peak area of the components appeared the characteristic fingerprint. CONCLUSION:The method and the fingerprint have a good reproducibility,and can be used to identify and evaluate the quality of processed Rhizome curcumae longae.
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AIM: To study fingerprint and characteristic components of the essential oil of processed Rhizoma Kaempferiae in order to control quality. METHODS: The fingerprint and characteristic components of the essential oil of 10 samples were determined by GC-MS. RESULTS: trans-Ethyl-p-methoxycinnamate,cis-Ethyl-p-methoxycinnamate,trans-Ethylcinnamate,n-Pentadecane,3-Carene,Borneol, 8-Heptadecene,Cyperene,Heptadecane,Globulol,Eucalyptol,cis-Methyl-p-methoxycinnamate and Eucarvone were gained in 10 samples,and relative contents of 13 components added up to 94.5%-98.2% of the total essential oil.The retention time and peak area of 13 components had built the characteristic fingerprint of essential oil of processed Rhizoma Kaempferiae. CONCLUSION: The commercial samples collected from various sources shows a strong similarity.The method has a good repeatability and can be used to control quality of processed Rhizoma Kaempferiae.